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Pea IE vesicles were solubilized in 5% digitonin and subjected to 2D BN/ SDS-PAGE
Semi-quantitative analysis of Tic20 and Tic110 on protein level was performed using immunoblots of envelope membranes isolated from two-week-old pea and four week-old Arabidopsis plants. In parallel, calibration curves were generated using a series of known concentrations of over expressed and purified proteins . After quantification of immunoblots from envelopes, amounts of PsTic20, PsTic110, AtTic20 and AtTic110…
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Agricultural activity can sometimes be restricted by ESA designation in very specific ways
Rapid urban and agricultural development in the region contributed to the decline of the gnatcatcher population, and it was listed as a federally threatened species in 1993. In April 2003, USFWS designated almost 500,000 acres of critical habitat for the gnatcatcher in Los Angeles, Orange, Riverside, San Bernardino, San Diego, and Ventura counties. Sunding’s analysis…
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Nutrient-rich water is pumped over a shallow trough that is lined with plastic or cement
For maximum benefit, vegetative buffers should be sized for the treatment area based on the expected runoff volumes for a particular operation . In short, Liu et al. reviewed 80 experiments, and meta-analyses of these data indicated that sediment removal was maximized with a 10-m buffer width and 9% slope, regardless of the ratio of buffer…