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Pea IE vesicles were solubilized in 5% digitonin and subjected to 2D BN/ SDS-PAGE
Semi-quantitative analysis of Tic20 and Tic110 on protein level was performed using immunoblots of envelope membranes isolated from two-week-old pea and four week-old Arabidopsis plants. In parallel, calibration curves were generated using a series of known concentrations of over expressed and purified proteins . After quantification of immunoblots from envelopes, amounts of PsTic20, PsTic110, AtTic20 and AtTic110…
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The insects were in colony for less than one year at the time of the experiments
The microbial community becomes predominately Lactobacillaceae for sixth instars and pupae. Once the insects reach the adult stage their most predominant family is Pseudomonadaceae. This pattern holds in the acetaminophen and caffeine treatment groups as well. Interestingly, the other treatment groups do not share this pattern. For antibiotic- and hormone-treated T. ni, Lactobacillaceae is the…
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Contamination from PPCPs also has the potential to negatively impact plants
Inhibitory potency increases several-fold when the glucosinolate alkyl chain gets longer , suggesting that PBITC, with its elongated alkyl chain compared to PEITC, may contribute an additional health benefit to this super food, although this remains to be proven. It is evident that watercress is particularly well-suited for indoor hydroponic growing systems, where plants exhibited…
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Even isolates of the same species can vary in response to N availability
Hyphal length rarely, if ever, decreases. In addition, preliminary evidence indicates that soil organic matter associated with mycorrhizal hyphae might become more prevalent. However, few data exist regarding changes in life span or decomposition rate under CO# enrichment, and these processes have strong controls over the long-term accumulation of soil organic matter pools over time.…
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The only significant pathway for nitrogen loss would have been through microbial growth
Riverside Native Plant treatments recovered on average 96% of the nitrogen tracer spike, and Motte Native Plant modules recovered 78%. In Invasive Plant modules at both sites, the above ground plant material held the majority of the total nitrogen within the plant . The Native Plant treatments at both sites exhibited less variable, smaller ratios of…
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One of the most important invertebrates in agricultural fields is earthworm
The land application of TWW and bio-solids can introduce contaminants of emerging concern into terrestrial environments . Consequently, a range of CECs have been detected in agricultural soils . Literature pertaining to the effects of CECs in terrestrial ecosystems is, however, limited . The majority of previous research has been concerned with the fate and effects…
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The effects of CECs in terrestrial organisms have garnered recent attention
Collectively, our findings provide genetic and physiological evidence that AVP1 is a new component required for plant growth under high external Mg2+ concentrations and functions in regulating Mg2+ tolerance via PPi hydrolysis. For on-plate growth assays, seeds of different genotypes were sterilized with 75% ethanol for 10 min, washed in sterilized water for three times,…
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Labor and its outcomes are central components of justice and urban agriculture
The Garden and Save the Farm . This trend is slowly infiltrating Southeastern San Diego. Mt. Hope Community Garden recently found out its property was being sold when a for-sale sign showed up on their fence. Elizabeth Studebaker, Neighborhood Investment Manager at the City of San Diego notes that the property “historically had been dedicated for…
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Actor-network theory has undoubtedly been useful in the study of geographies of food
The corporate food regime uses this “commodity fetishism” to hide the socio-natural relations of productions that might dissuade consumers and inhibit endless capital accumulation . Work in economic geography on global commodity chains and circuits has sought to “lift the veil” on the secret lives of commodities in the apparel and agri-food industries . This work seeks…
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Using the genomic clones as templates we obtained the full-length coding region by overlap PCR
We also observed a significant increase in the transcripts of the JA-regulated genes PLETHORA 1 , PLT3 and PLT5 in 1RW and UBI::OPRIII-R5 relative to 1RS at 16 DAG but not at 6 DAG . These results support a role of the OPRIII genes on the JA-Ile signaling pathway, which is known to be involved…